限制性内切酶;
It enables a specific gene to be located on a particular restriction enzyme fragment.
它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。
来源:互联网摘选Rapid Preparation and Restriction Enzyme Analysis of the DNA of Canine Adenovirus Type 2
犬Ⅱ型腺病毒DNA的快速提取及限制性酶切分析
来源:互联网摘选采用PCR法和限制性内切酶分析对12种分枝杆菌进行鉴定
来源:互联网摘选The recombined clone was confirmed by restriction enzyme and sequencing analysis.
将获得基因片段重组到pCI质粒中,转化到大肠杆菌Top10后挑选克隆,利用限制性酶切和核苷酸序列分析鉴定重组质粒。
来源:互联网摘选Restriction Enzyme Mediated Integration, a New Strategy for Cloning New Genes
限制酶介导整合技术:一种克隆新基因的新策略
来源:互联网摘选Gene mappings were made in 11 cases among 25 immuno-positives with restriction enzyme.
从25例中随机抽取11例作限制性内切酶解的基因图谱分析,以对照检查酶联免疫吸附试验法的准确度,结果10例为α-地贫1,基因型是αα/一一;
来源:互联网摘选各扩增产物长度和限制性酶酶切结果表明均为预期产物。
来源:互联网摘选孕妇及新生儿巨细胞病毒感染的聚合酶链反应及限制酶分析
来源:互联网摘选Recombinants were confirmed by colony PCR and restriction enzyme digestion.
经菌落PCR和质粒酶切鉴定后,将目的基因进行序列测定。
来源:互联网摘选In vitro Enlargement of pBR322 and the Related Restriction Enzyme Maps
质粒pBR322的体外扩建以及有关限制性核酸内切酶图谱
来源:互联网摘选Restriction enzyme pattern analysis of the DNAs of three nuclear polyhedrosis viruses
三种昆虫核型多角体病毒DNA的限制性内切酶酶解分析
来源:互联网摘选And the results were further confirmed by restriction enzyme analysis of the genomic DNA fragments.
限制性酶切分析其基因组DNA。
来源:互联网摘选PCR产物10.0μL, 加入2.0μL10×Buffer, 83.350nkat内切酶,7.5μL双蒸水构成20μL酶 切反应体系.
来源:互联网摘选To verified the by restriction enzyme digestion, bacterial colony PCR, and sequencing analysis.
并对重组质粒进行双酶切、菌落PCR及测序分析进行鉴定。
来源:互联网摘选并经过PCR和酶切鉴定重组病毒构建成功。
来源:互联网摘选Ultrasensitive detection method for restriction enzyme cleavage product using molecular beacon.
建立了限制性内切酶切割产物的超灵敏检测方法。
来源:互联网摘选The mutation was confirmed by digestion of the PCR products with restriction enzyme.
发现突变后用特异性限制性内切酶对突变位点进行酶切,验证突变。
来源:互联网摘选结果构建的表达载体经过限制性内切酶分析和DNA序列测定,证实准确无误。
来源:互联网摘选Restriction enzyme analysis of human papilloma virus ( hpv) dna from common warts
人乳头瘤病毒DNA的限制性核酸内切酶分析
来源:互联网摘选The DNA vaccine was identified by restriction enzyme analysis, sequencing and expression.
对此疫苗进行酶切、测序和表达鉴定。
来源:互联网摘选用限制性内切酶酶切的方法判断该检测技术的准确性。
来源:互联网摘选The construct was confirmed by PCR, restriction enzyme digestion and direct sequencing.
经限制性内切酶酶切和测序证实。
来源:互联网摘选Restriction enzyme mapping and SDS-PAGE protein analysis were done on four transformants.
还进行了限制酶酶切分析和蛋白质SDS-PAGE分析。
来源:互联网摘选实验结果还表明,REMI的转化效率是一般质粒转化效率的19.12~22.72倍。
来源:互联网摘选The recombinants were analyzed and identified by restriction enzyme, PCR and sequencing.
通过酶切、PCR及测序鉴定各重组体。
来源:互联网摘选
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